Journal: Nature Microbiology
Article Title: Vaccine plus microbicide effective in preventing vaginal SIV transmission in macaques
doi: 10.1038/s41564-023-01353-7
Figure Lengend Snippet: Expression of a ) OX40, b ) CD40L, c ) CD69, d ) Ki67, e ) LAG3, f ) CTLA-4, g ) PD-1 and h ) PDL-1 in Th1 cells upon stimulation in the vaccine+SAMT-247 group (n = 4) and vaccine group (n = 2). Data shown in ( a-h ) were analyzed with the two-tailed Wilcoxon signed-rank test. Horizontal and vertical bars denote mean and SD.
Article Snippet: PBMCs were stained for 30 min at room temperature with the following: LIVE/DEAD Fixable Blue Dead Cell Stain (cat. no. L23105, Thermo Fisher); BV786 anti-CD45 (D058-1283; cat. no. 563861, 5 μl), BUV737 anti-CD3 (SP34-2; cat. no. 741872, 5 μl), BV711 anti-CD4 (L200; cat. no. 740807, 5 μl), BUV496 anti-CD8 (RPA-T8; cat. no. 612942, 5 μl), PE-CF594 anti-PDL1 (MIH1; cat. no. 563742, 5 μl), BB700 anti-CTLA-4 (BNI3; cat. no. 566901, 5 μl), PE-Cy5 anti-OX40 (CD134) (ACT35; cat. no. 551500, 5 μl), BUV563 anti-CD40L (CD154) (24-31; cat. no. 752854, 5 μl), PE-Cy7 anti-CD69 (FN50; cat. no. 557745, 5 μl), PE anti-CD95 (DX2; cat. no. 555674, 5 μl) from BD Bioscience; FITC anti-LAG3 (3DS223H; cat. no. 369326, 5 μl) from Thermofisher; BV750 anti-PD1 (EH12.2H7; cat. no. 329966, 5 μl), Alexa 700 anti-CXCR3 (G025H7; cat. no. 353742, 5 μl), BV605 anti-CCR6 (G034E3; cat. no. 353420, 5 μl) from BioLegend.
Techniques: Expressing, Two Tailed Test